Novel RpoS-Dependent Mechanisms Strengthen the Envelope Permeability Barrier during Stationary Phase
نویسندگان
چکیده
منابع مشابه
The Mutation of the rpoS Gene, the Central Regulator of Stationary Phase, Affects the Cell Division in Flexibacter chinensis
A one kb portion of the rpoS gene from Flexibacter chinensis was isolated by PCR, sequenced and compared to the rpoS gene of a variety of other organisms. The gene was found to be 98% similar to previously sequenced genes. Mutation of the rpoS gene with tri-parental mating produced strain JR101 and the growth rate of the mutant was compared with that of the wild-type. The mutant grew slower, an...
متن کاملAltered stationary-phase response in a Borrelia burgdorferi rpoS mutant.
The homolog of the chromosomally encoded stationary-phase sigma factor RpoS in Borrelia burgdorferi was inactivated using gyrB(r) as a selectable marker. Two-dimensional nonequilibrium pH gradient electrophoresis of stationary-phase cell lysates identified at least 11 differences between the protein profiles of the rpoS mutant and wild-type organisms. Wild-type B. burgdorferi had a growth phase...
متن کاملRole of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.
This work investigated the role of rpoS in the development of increased cell envelope resilience and enhanced pressure resistance in stationary-phase cells of Escherichia coli. Loss of both colony-forming ability and membrane integrity, measured as uptake of propidium iodide (PI), occurred at lower pressures in E. coli BW3709 (rpoS) than in the parental strain (BW2952). The rpoS mutant also rel...
متن کاملIdentification of conserved, RpoS-dependent stationary-phase genes of Escherichia coli.
During entry into stationary phase, many free-living, gram-negative bacteria express genes that impart cellular resistance to environmental stresses, such as oxidative stress and osmotic stress. Many genes that are required for stationary-phase adaptation are controlled by RpoS, a conserved alternative sigma factor, whose expression is, in turn, controlled by many factors. To better understand ...
متن کاملthe mutation of the rpos gene, the central regulator of stationary phase, affects the cell division in flexibacter chinensis
a one kb portion of the rpos gene from flexibacter chinensis was isolated by pcr, sequenced and compared to the rpos gene of a variety of other organisms. the gene was found to be 98% similar to previously sequenced genes. mutation of the rpos gene with tri-parental mating produced strain jr101 and the growth rate of the mutant was compared with that of the wild-type. the mutant grew slower, an...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2017
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00708-16